Abstract: The extraction process of lutein algae powder was studied by enzyme extraction, the affecting factors of lutein extraction such as different solvents, different kinds of enzyme, enzyme quantity, enzymolysis time and stirring time were investigated in single factor experiments. An orthogonal test table L9(33) was used to optimize the affecting factors on the base of single factor experiments. The content of lutein in the end product was determined. The results showed that the optimal extraction and saponification parameter are as follows, the enzyme liquid adding 5 mL, hydrolysis time 2.5 h, magnetic stirring time 2.5 h. Under this condition, the yield of lutein reached 2.18 mg/g.