Pretreatment is the most important step during biochemical conversion of lignocellulose to biofuels and biochemicals. However, lack of mixing in the conventional pretreatment process resulted in the deficiency of mixing, heat and mass transfer, the non conformity of the pretreatment intensity, and thus led to the partial insufficient of lignocellulose structure decomposition, and partial excessive decomposition of the lignocellulose structure to generate inhibitors, which in turn impeded the subsequent enzymatic hydrolysis and microbial fermentation. This study aims to demonstrate the viability of introducing helical impeller mixing into the dry dilute sulfuric acid pretreatment process. Firstly, considering the high ability of water adsorption and the resulted difficulty of rheologcial property characterization of the corn stover system, the rheological property parameters of flow index (n) and the consistency coefficient (Kpl) were obtained through calculating the apparent viscosity (ηa) and the apparent shearing rate (γeff) of the corn stover system according to the power law model. Furthermore, CFD models of the dry dilute sulfuric acid pretreatment in a helical stirring bioreactor using the obtained rheological property parameters were constructed, and the CFD simulation results were in agreement with the cold mold results in three different scale bioreactors. The results of both CFD simulation and cold mold experiments showed that the practicability of introducing helical impeller into the dry dilute sulfuric acid pretreatment bioreactor.