Abstract:
Small caliber vascular grafts of expanded polytetrafluoroethylene (ePTFE) is one of the most common substitutes in vascular implantation surgery. However, late thrombosis and in vascular restenosis constitute the crucial factors that lead to long term implantation failure. Thrombosis inhibition can be achieved by buliding up CD133 functionalized heparin collagen (HEP/COL) multilayer, which also provides possibilities of the vascular re endothelialization. The HEP/COL multilayer is constructed via layer by layer assembly technique and the assembly process is monitored by Fourier transform infrared spectrum (FT IR), scanning electron microscrope(SEM). Confocal laser scanning microscropy is used for characterizing the successful immobilization of CD133 antibody. Finally, the CD133 antibody functionalized HEP/COL multilayer was successfully coated on the ePTFE vascular surface. The result of contact angle experiment reveals great improvement in hydrophilicity, while water contact angle decreasing from 127° to 106°. The in vitro platelet adhension rate decreases dramatically after modification of the ePTFE vascular surface, indicating improved anticoagulant property.