Lignocellulose biorefining has great potentials of feedstock supply and product market in the major agricultural countries. The flowsheet simulation model for the industrial scale biorefining process of cellulosic ethanol production using lignocellulosic feedstocks was established. The physical property database was established based on the materials analysis and parameter estimation methods for the biomass components involved in the lignocellulose processing. The biorefining plant with the processing capacity of 300 000 t corn stover annual for production of fuel ethanol was simulated using the established Aspen plus model and the water and energy consumption of the processes was analyzed. The whole flowsheet balance of the mass and enthalpy data were calculated and used for the design of the processes. The model can be used in the future industrial plants for the process design, operation optimization and economic evaluation.