Abstract: Because of the uncertainties in the network itself and the application nodes, NCS usually works in a variable loads, open and unstable environment. To ensure the stability of the NCS in application, this paper presents a strategy of variable sampling periods based on multiple input and multiple output support vector machine (MIMOSVM). This proposed strategy dynamically update the sampling period of the control loop, according to the network load changing. Simulation results show that this present method may ensure the stability of NCSs with variable loads, and enhance the schedule ability of the network.