Under the suitable conditions of synthesis reaction, n dodecyl glucoside products were prepared by glucose and n dodecanol by the direct method. Glucoside products were also investigated and characterized by FAB, GC, 1H NMR and IR. According to the results of above mentioned experiment, polymerization( n ) and HLB (hydrophilic lipophilic balance) of n dodecyl glucoside products were obtained by calculation. In addition, surface properties of products, such as surface tension, critical micell concentration, emulsifying property, solubilizing property on organic solvents and foaming property and stability and so on, were also surveyed. The experimental results show that n dodecyl glucoside is a kind of excellent non ionic surfactant, which possesses high foaming power and stability, emulsifying, softening detergent, low irritating properties and high solubilizing properties on organic solvents.