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    汤军芝, 冯天才, 刘世友, 冯耀宇. 菌群降解菲和氧化三价砷的双功能验证[J]. 华东理工大学学报(自然科学版), 2013, (4): 450-456.
    引用本文: 汤军芝, 冯天才, 刘世友, 冯耀宇. 菌群降解菲和氧化三价砷的双功能验证[J]. 华东理工大学学报(自然科学版), 2013, (4): 450-456.
    TANG Jun-zhi, FENG Tian-cai, LIU Shi-you, FENG Yao-yu. Functional Verification of A Dual functional Consortium: Degrading Phenanthrene and Oxidizing Arsenite Simultaneously[J]. Journal of East China University of Science and Technology, 2013, (4): 450-456.
    Citation: TANG Jun-zhi, FENG Tian-cai, LIU Shi-you, FENG Yao-yu. Functional Verification of A Dual functional Consortium: Degrading Phenanthrene and Oxidizing Arsenite Simultaneously[J]. Journal of East China University of Science and Technology, 2013, (4): 450-456.

    菌群降解菲和氧化三价砷的双功能验证

    Functional Verification of A Dual functional Consortium: Degrading Phenanthrene and Oxidizing Arsenite Simultaneously

    • 摘要: 从上海某含菲和砷复合污染物的焦化厂土壤中富集、分离得到了一个双功能菌群。在含200 mg/L菲和60 mg/L As2O3的培养基中,该菌群48 h内能降解71.4%的菲和氧化96.2%的三价砷。从分子水平进行功能验证,选择菲降解基因(RHDα)和砷氧化基因(aoxB)构建文库,基因RHDα与Pseudomonas aeruginosa和Pseudomonas putida基因的同源性较高;而基因aoxB与Pseudomonas stutzeri和Alcaligenes faecalis的同源性较高。采用实时荧光定量PCR技术定量2个基因,在48 h内,1 mL菌液中aoxB基因拷贝数为3.00×108~1.48×1010,而RHDα则为2.54×107~8.70×109。该菌群将在含多环芳烃和砷复合污染场地中具有潜在的应用价值。

       

      Abstract: A heterotrophic bacterial consortium, degrading Phenanthrene(PHE) and oxidizing As(Ⅲ) simultaneously, was isolated from the soil in a discarded coking plant containing PHE and As(Ⅲ). After 48 h incubation, the bacterial consortium removed 71.4% of PHE and transformed 96.2% of As(Ⅲ) in liquid culture containing 200 mg/L PHE and 60 mg/L As2O3 . To validate the dual function of the consortium from molecular level, phenanthrene degrading gene RHDα and arsenite oxidizing gene aoxB were chosed to establish clone libraries. The RHDα genes had high homology with Pseudomonas aeruginosa and Pseudomonas putida, while aoxB genes had high homology with Pseudomonas stutzeri and Alcaligenes faecalis. Real time quantitative PCR results showed that the copy numbers of aoxB genes increased from 3.00×108 to 1.48×1010, while the copy numbers of RHDα genes increased from 2.54×107 to 8.70×109 after 48 h incubation. The dual function consortium showed potentials for practical applications of degrading phenanthrene and arsenic co contamination.

       

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