Abstract: Abstract: High efficient extraction of lipid from chlorella based on double solvent method was conducted in this study. Components of oil and contents of both lipid and water soluble components were analyzed. The effects of solvent system, addition order, volume ratio and solid to liquid ratio (ratio of mass of the chiorella (g) and volume of the mixed solvent (mL)) on extraction efficiency were compared. The results indicated that 45.9% extractive and 16.1% lipid (of dry chlorella powder) could be extracted on the condition of petroleum ether and ethanol as extraction agents with a volume ratio of 1∶2, non polar solvent adding before polar solvent, solid to liquid ratio of 1∶20.