Abstract: Polycarbonate polyurethane with pendant carboxyl groups were successfully synthesized using polycarbonate diols (PCDL) as soft segments, diphenylmethane diisocyanate (MDI) as hard segments, and 2, 2-bis(hydroxymethyl) propionic acid (bis-MPA) as chain extenders. Heparin was immobilized onto the surface of polyurethane through a covalent reaction in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDAC). The surface exposed heparin content was measured to be 1.92 μg/cm2 and the hydrophilicity of the heparinized polymer was greatly improved. The capability of adsorbing protein decreased after heparin immobilization. Animal implantation results showed good biocompatibility of the heparin immobilized material and this novel polymer could be applied as a potential coating material for coronary stents with lower inflammatory reaction formation than the original material.