Abstract: Luteolin is a natural flavonoid with remarkable clinical potential, yet its applications are limited by poor solubility and bioavailability. This study developed a novel toroidal microfluidic device to construct tunable luteolin-loaded liposomal nanoparticles. The optimized nanoparticles exhibited 220.5 nm diameter, 64.72% encapsulation efficiency, and excellent monodispersity (PDI=0.037). In vitro studies demonstrated effective cellular membrane penetration for intracellular delivery, offering new perspectives for lipophilic drug delivery system design.