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    王萌, 张爽, 王靖, 曹雪华, 岳述荣, 刘昌胜. 载rhBMP-2聚乳酸/磷酸钙支架在兔脊柱融合中的应用[J]. 华东理工大学学报(自然科学版), 2023, 49(6): 787-793. DOI: 10.14135/j.cnki.1006-3080.20221009002
    引用本文: 王萌, 张爽, 王靖, 曹雪华, 岳述荣, 刘昌胜. 载rhBMP-2聚乳酸/磷酸钙支架在兔脊柱融合中的应用[J]. 华东理工大学学报(自然科学版), 2023, 49(6): 787-793. DOI: 10.14135/j.cnki.1006-3080.20221009002
    WANG Meng, ZHANG Shuang, WANG Jing, CAO Xuehua, YUE Shurong, LIU Changsheng. A Polylactic Acid/Calcium Phosphate Scaffold with rhBMP-2 for Spinal Fusion in Rabbit[J]. Journal of East China University of Science and Technology, 2023, 49(6): 787-793. DOI: 10.14135/j.cnki.1006-3080.20221009002
    Citation: WANG Meng, ZHANG Shuang, WANG Jing, CAO Xuehua, YUE Shurong, LIU Changsheng. A Polylactic Acid/Calcium Phosphate Scaffold with rhBMP-2 for Spinal Fusion in Rabbit[J]. Journal of East China University of Science and Technology, 2023, 49(6): 787-793. DOI: 10.14135/j.cnki.1006-3080.20221009002

    载rhBMP-2聚乳酸/磷酸钙支架在兔脊柱融合中的应用

    A Polylactic Acid/Calcium Phosphate Scaffold with rhBMP-2 for Spinal Fusion in Rabbit

    • 摘要: 脊柱融合术是治疗脊柱退变性疾病的常用方法,提高植骨材料的成骨活性对提高脊柱融合效果具有重要意义。重组人骨形态发生蛋白-2(rhBMP-2)是一种促进新骨生成的生长因子,能够快速诱导骨修复。将rhBMP-2通过物理吸附负载于聚乳酸/磷酸钙多孔支架表面,再将表面涂覆羟丙甲基纤维素(HPMC)涂层,得到HPMC涂层改性的载rhBMP-2聚乳酸/磷酸钙支架。rhBMP-2的负载使支架具有诱导成骨活性,而HPMC涂层能够使rhBMP-2的释放减缓,有利于促进新骨生成。将该支架植入兔脊柱84 d后发现脊柱椎体间生成了连续的骨组织,达到了骨性融合,因此HPMC改性的载rhBMP-2聚乳酸/磷酸钙支架是一种可用于脊柱融合植骨的理想材料。

       

      Abstract: Spinal fusion is a commonly used method for the treatment of spine degenerative diseases. To achieve spine fusion successfully, the osteogenic activity of bone-graft materials needs to be high enough to promote bony fusion. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a growth factor that promotes new bone formation and induces bone repair rapidly. In this study, a porous polylactic acid/calcium phosphate scaffold was prepared, to which rhBMP-2 was loaded by physical adsorption to make the scaffold osteoinductive. Furthermore, the rhBMP-2-loaded scaffold was coated with hydroxypropyl methylcellulose (HPMC) for the sustained release of rhBMP-2. The HPMC coated scaffold bearing rhBMP-2 was implanted in rabbit spines. We observed a continuous bone tissue generation at 12 weeks post-operation, and the spines were bony fused. Our results suggest the promise of using the HPMC-coated polylactic acid/calcium phosphate scaffold bearing rhBMP-2 as a bone-repairing material for spinal fusion.

       

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