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  • CN 31-1691/TQ

不同碳源对微生物反硝化性能的影响

楼超楠 韩昫身 金艳 何焱 宋兴福

楼超楠, 韩昫身, 金艳, 何焱, 宋兴福. 不同碳源对微生物反硝化性能的影响[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220524001
引用本文: 楼超楠, 韩昫身, 金艳, 何焱, 宋兴福. 不同碳源对微生物反硝化性能的影响[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220524001
LOU Chaonan, HAN Xushen, JIN Yan, HE Yan, SONG Xingfu. Effect of Different Carbon Sources on Microbial Denitrification Performance[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220524001
Citation: LOU Chaonan, HAN Xushen, JIN Yan, HE Yan, SONG Xingfu. Effect of Different Carbon Sources on Microbial Denitrification Performance[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220524001

不同碳源对微生物反硝化性能的影响

doi: 10.14135/j.cnki.1006-3080.20220524001
基金项目: 上海市青年科技英才扬帆计划(20YF1409500);中国博士后科学基金特别资助及面上资助(2021T140206,2021M691010);中国石油科技创新基金(2020D-5007-0502)
详细信息
    作者简介:

    楼超楠(1996—),女,绍兴人,硕士生,研究方向为废水生物脱氮技术。E-mail: y30191203@mail.ecust.edu.cn

    通讯作者:

    宋兴福,E-mail:xfsong@ecust.edu.cn

  • 中图分类号: X703

Effect of Different Carbon Sources on Microbial Denitrification Performance

  • 摘要: 采用序批式反应器,研究了甲醇、乙酸钠、乙二醇、丙三醇和葡萄糖五种碳源对不同电子受体反硝化性能的影响。结果表明以NO3−N及NO2−N为电子受体时,甲醇、乙酸钠、乙二醇、丙三醇、葡萄糖的最佳碳氮比分别为5、5、7、7、8与3、3、3.5、4、4,比反硝化速率从快到慢依次是乙酸钠、甲醇、乙二醇、丙三醇、葡萄糖与甲醇、乙酸钠、乙二醇、葡萄糖、丙三醇。经成本计算可得,处理相同浓度的NO3−N和NO2−N,需要投加的碳源成本从低到高依次为甲醇、葡萄糖、乙酸钠、乙二醇、丙三醇和甲醇、葡萄糖、乙二醇、乙酸钠、丙三醇。本文为低碳氮比废水处理过程提供碳源优选参考。

     

  • 图  1  不同碳氮比甲醇在稳定周期内对氮素的去除效果

    Figure  1.  Effect of methanol on nitrogen removal at different C/N ratio in stable period

    (a: NO3−N removal embedded TN removal in the NO3−N influent system; b: NO2−N removal in the NO2−N influent system)

    图  2  不同碳氮比乙酸钠在稳定周期内对氮素的去除效果

    Figure  2.  Effect of sodium acetate on nitrogen removal at different C/N ratio in stable period

    (a: NO3−N removal embedded TN removal in the NO3−N influent system; b: NO2−N removal in the NO2−N influent system)

    图  3  不同碳氮比乙二醇在稳定周期内对氮素的去除效果

    Figure  3.  Effect of glycol on nitrogen removal at different C/N ratio in stable period

    (a: NO3−N removal embedded TN removal in the NO3−N influent system; b: NO2−N removal in the NO2−N influent system)

    图  4  不同碳氮比丙三醇在稳定周期内对氮素的去除效果

    Figure  4.  Effect of glycerol on nitrogen removal at different C/N ratio in stable period

    (a: NO3−N removal embedded TN removal in the NO3−N influent system; b: NO2−N removal in the NO2−N influent system)

    图  5  不同碳氮比葡萄糖在稳定周期内对氮素的去除效果

    Figure  5.  Effect of glucose on nitrogen removal at different C/N ratio in stable period

    (a: NO3−N removal embedded TN removal in the NO3−N influent system; b: NO2−N removal in the NO2−N influent system)

    表  1  反应器运行各阶段电子受体及C/N

    Table  1.   Electron acceptors and C/N ratios at each stage of operation in five reactors

    Phase
    (d)
    Electron acceptorsC/N
    Methanol
    Sodium
    acetate
    GlycolGlycerolGlucose
    Ⅰ(0−13)NO3−N33333
    Ⅱ(14−18)44444
    Ⅲ(19−23)55555
    Ⅳ(24−27)66666
    Ⅴ(28−32)77777
    Ⅵ(33−38)77778
    Ⅶ(40−47)NO2−N22222
    Ⅷ(48−53)2.52.52.52.52.5
    Ⅸ(54−58)33333
    Ⅹ(59−63)3.53.53.53.53.5
    Ⅺ(64−68)///44
    下载: 导出CSV

    表  2  不同碳源条件下反硝化理论碳氮比

    Table  2.   Theoretical C/N ratio of denitrification under different carbon sources

    Carbon sourceChemical reaction equation of denitrificationMolar ratio
    (carbon/nitrogen)
    Theoretical COD of
    carbon source
    Theoretical C/N ratio
    Methanol${\rm{5C{H_3}OH + 6NO_3^ - + 6{H^ + } \to 3{N_2} + 5C{O_2} + 13{H_2}O}}$0.84482.86
    Sodium acetate${\rm{ 5C{H_3}COOH + 8NO_3^ - + 8{H^ + } \to 4{N_2} + 10C{O_2} + 14{H_2}O}}$0.63642.86
    Glycol${\rm{{ {C} _2}{H_6}{O_2} + 2NO_3^ - + 2{H^ + } \to {N_2} + 2C{O_2} + 4{H_2}O}}$0.50802.86
    Glycerol${\rm{5{C_3}{H_8}{O_3} + 14NO_3^ - + 14{H^ + } \to 7{N_2} + 15C{O_2} + 27{H_2}O}}$0.361122.86
    Glucose${\rm{5{C_6}{H_{12} }{O_6} + 24NO_3^ - + 24{H^ + } \to 12{N_2} + 30C{O_2} + 42{H_2}O}}$0.211922.86
    Methanol${\rm{{ {CH} _3}OH + 2NO_2^ - + 2{H^ + } \to {N_2} + C{O_2} + 3{H_2}O }}$0.50481.71
    Sodium acetate${\rm{3C{H_3}COOH + 8NO_2^ - + 8{H^ + } \to 4{N_2} + 6C{O_2} + 10{H_2}O}}$0.38641.71
    Glycol${\rm{3{C_2}{H_6}{O_2} + 10NO_2^ - + 10{H^ + } \to 5{N_2} + 6C{O_2} + 14{H_2}O}}$0.30801.71
    Glycerol${\rm{3{C_3}{H_8}{O_3} + 14NO_2^ - + 14{H^ + } \to 7{N_2} + 9C{O_2} + 19{H_2}O}}$0.211121.71
    Glucose${\rm{{ {C} _6}{H_{12} }{O_6} + 8NO_2^ - + 8{H^ + } \to 4{N_2} + 6C{O_2} + 10{H_2}O}}$0.161921.71
    Note: Molar ratio represents the moles of carbon source for conversion of 1 mole NOX−N in denitrification process. Theoretical COD of carbon source represents theoretical COD of 1 mole carbon source
    下载: 导出CSV

    表  3  不同碳源投加量下各SBR反应器内比反硝化速率

    Table  3.   Specific denitrification rates (SDNR) obtained with 5 different carbon sources

    MethanolSodium acetateGlycolGlycerolGlucose
    SDNR (mg/(g·h))
    NO3−NC/N=35.4(9.1)3.6(7.4)2.9(7.3)1.8(4.4)1.5(3.9)
    C/N=45.5(11.2)4.2(9)3.5(7.7)2.1(4.1)1.6(4.9)
    C/N=56.2(11.8)7.7(9.8)4.2(8.5)2.5(5.4)2.0(5.3)
    C/N=66.6(12.8)7.7(10.7)4.4(9)2.9(7.4)2.4(5.4)
    C/N=76.7(15.3)8.2(11.3)4.4(9.2)4.0(8.4)2.8(6.7)
    C/N=8////3.7(6.7)
    NO2−NC/N=24.5(6.7)3.2(5.5)2.6(4.2)2.0(3.8)2.3(3.9)
    C/N=2.55.7(8.3)3.9(6.2)3.2(5)2.9(5.6)2.8(4.4)
    C/N=36.2(8.7)5.4(7.3)4.2(5.9)3.0(5.8)3.3(5.6)
    C/N=3.56.3(10.5)7.1(8.4)5.1(6.5)3.6(7.1)3.8(6.1)
    C/N=4///3.7(7.1)3.9(6.1)
    Note: The datas in bracket are the maximum specific denitrification rates
    下载: 导出CSV

    表  4  不同碳源在最佳碳氮比下的成本分析表

    Table  4.   Cost of carbon sources at corresponding optimal C/N ratio for nitrogen removal

    Electron acceptorOptimal C/N ratioMass ratio of carbon source to TNPrice converted to100%
    purity (yuan/ton)
    TN (mg/L)Carbon source (mg/L)Cost of carbon source (yuan/m3 water)
    MethanolNO3−N5.03.32995501650.50
    NO2−N3.02.0501000.30
    Sodium acetateNO3−N5.06.45200503201.66
    NO2−N3.03.8501900.98
    GlycolNO3−N7.05.56100502751.68
    NO2−N3.52.7501350.82
    GlycerolNO3−N7.05.713350502853.80
    NO2−N4.03.3501652.20
    GlucoseNO3−N8.07.63690503801.40
    NO2−N4.03.8501900.70
    Note: The prices of chemicals were used according to www.1688.com (November 2021).
    下载: 导出CSV
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  • 收稿日期:  2022-05-24
  • 网络出版日期:  2022-09-09

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