Abstract: Serum amyloid A (SAA) is an acute-phase protein mainly produced by the liver in response to proinflammatory cytokines. SAA genes and proteins are significantly activated during the acute phase response, which comprises a number of phenomena that occur in the presence of inflammation and infection, increased temperature and hormonal and metabolic alterations. Therefore, SAA is a sensitive indicator of inflammation in the early stage of infectious diseases, which is important for diagnosis, evaluation, monitoring and treatment of inflammation. Fluorescent immunochromatography is one of the most popular strategies for point-of-care testing (POCT), which is capable of rapid screening for disease detection. Fluorescent microspheres QM-OH@PS-COOH were obtained from aggregation-induced emission (AIE) quinoline-malononitrile (QM) derivatives QM-OH. The morphology and structure of these QM fluorescent microspheres were characterized by scanning electron microscopy et al. Finally, these fluorescent microspheres were utilized to detect for SAA concertation in clinical samples via fluorescent immunochromatography. The results showed that QM-OH@PS-COOH had uniform sizes with regular shapes. Compared with commercial fluorescent microspheres, these AIE microspheres had similar density, solid content and carboxyl content. The QM-OH@PS-COOH system exhibited the detection of SAA with high sensitivity in clinical samples via fluorescent immunochromatography. Thus, this new type of QM fluorescent microspheres could be employed as an important tool for clinical diagnosis, enabling quantitatively analyze and monitor the concentration of SAA during the inflammation process. Therefore, we believe that these detection platforms based on QM-OH@PS-COOH can serve as a screening platform for early disease detection, especially self-testing in POCT.