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    周倍, 刘楠, 刘泽波, 庄英萍, 王泽建, 郭美锦. 三七悬浮细胞生物合成绿原酸类物质及其生物活性[J]. 华东理工大学学报(自然科学版), 2023, 49(4): 515-528. DOI: 10.14135/j.cnki.1006-3080.20220223001
    引用本文: 周倍, 刘楠, 刘泽波, 庄英萍, 王泽建, 郭美锦. 三七悬浮细胞生物合成绿原酸类物质及其生物活性[J]. 华东理工大学学报(自然科学版), 2023, 49(4): 515-528. DOI: 10.14135/j.cnki.1006-3080.20220223001
    ZHOU Bei, LIU Nan, LIU Zebo, ZHUANG Yingping, WANG Zejian, GUO Meijin. Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells[J]. Journal of East China University of Science and Technology, 2023, 49(4): 515-528. DOI: 10.14135/j.cnki.1006-3080.20220223001
    Citation: ZHOU Bei, LIU Nan, LIU Zebo, ZHUANG Yingping, WANG Zejian, GUO Meijin. Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells[J]. Journal of East China University of Science and Technology, 2023, 49(4): 515-528. DOI: 10.14135/j.cnki.1006-3080.20220223001

    三七悬浮细胞生物合成绿原酸类物质及其生物活性

    Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells

    • 摘要: 利用三七 (Panax notoginseng) 悬浮细胞生物合成绿原酸类物质 (CGAs),并对其生物活性进行了研究。对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力。结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30 μmol/L 茉莉酸甲酯诱导3 d后,CGAs产量 (684.07 mg/L) 比原工艺 (475.18 mg/L) 提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力。

       

      Abstract: In this paper, Panax notoginseng (P. notoginseng) suspension cells were used for the production of chlorogenic acids (CGAs), of which bioactivities were evaluated. Firstly, CGAs in P. notoginseng suspension cells were identified by liquid chromatography-tandem mass spectrometry. Secondly, the suspension culture system and elicitation mode were optimized. Ultimately, the antioxidant and α-glucosidase inhibitory activity of CGAs from P. notoginseng cells were determined. Four CGAs were inferred inP. notoginseng cells. The optimal condition was obtained (B5+40 g/L sucrose, pH 7.5 and 15% inoculation size). By co-culture with 30 μmol/L methyl jasmonate for 3 d, the CGAs yield could reach up to 684.07 mg/L, which was 1.44 times that of the control. CGAs from P. notoginseng cells showed an excellent antioxidant capacity in radical-scavenging test and, moreover, certain inhibition on α-glucosidase activity. Our results indicate that the production of CGAs by P. notoginseng cells has potential applications in healthy food and daily cosmetics.

       

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