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  • ISSN 1006-3080
  • CN 31-1691/TQ

三七悬浮细胞生物合成绿原酸类物质及其生物活性

周倍 刘楠 刘泽波 庄英萍 王泽建 郭美锦

周倍, 刘楠, 刘泽波, 庄英萍, 王泽建, 郭美锦. 三七悬浮细胞生物合成绿原酸类物质及其生物活性[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220223001
引用本文: 周倍, 刘楠, 刘泽波, 庄英萍, 王泽建, 郭美锦. 三七悬浮细胞生物合成绿原酸类物质及其生物活性[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220223001
ZHOU Bei, LIU Nan, LIU Zebo, ZHUANG Yingping, WANG Zejian, GUO Meijin. Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220223001
Citation: ZHOU Bei, LIU Nan, LIU Zebo, ZHUANG Yingping, WANG Zejian, GUO Meijin. Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220223001

三七悬浮细胞生物合成绿原酸类物质及其生物活性

doi: 10.14135/j.cnki.1006-3080.20220223001
基金项目: 国家重点研发计划 (2020YFA0906800)
详细信息
    作者简介:

    周倍:周 倍 (1996—),硕士生,研究方向为植物细胞培养与代谢工程。E-mail: zhoubei1108@163.com

    通讯作者:

    郭美锦,E-mail: guo_mj@ecust.edu.cn

  • 中图分类号: TS201.3

Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells

  • 摘要: 利用三七 (P. notoginseng) 悬浮细胞生物合成绿原酸类物质 (CGAs),并对其生物活性进行了研究。对三七细胞中的CGAs进行定性分析;优化了细胞培养条件以及6种诱导模式;测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶抑制活性。主要结果为:分析了4种CGAs;最优工艺条件为:B5 + 4.0 mg/L NAA + 0.2 mg/L 6-BA + 40 g/L蔗糖 + 2 g/L PVP,pH 7.5,15%接种量;经30 μmol/L 茉莉酸甲酯诱导3 d后,CGAs产量比原工艺提高了0.44倍 (684.07 mg/L);三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力。

     

  • 图  1  三七愈伤组织中绿原酸 (CGAs) HPLC与UPLC-MS/MS分析

    Figure  1.  Detection of CGAs from P. notoginseng calli

    图  2  三七愈伤组织中CGAs裂解过程

    Figure  2.  Deduced fragmentation pathways of CGAs from P. notoginseng calli

    图  3  三七悬浮细胞培养体系的建立

    Figure  3.  Development of P. notoginseng suspension cell culture system

    图  4  不同培养条件对三七悬浮细胞生物量及CGAs产量影响

    Figure  4.  Biomass and CGAs yield of P. notoginseng suspension cells grown under different conditions

    图  5  三七细胞悬浮培养参数

    Figure  5.  Culture parameters of P. notoginseng suspension cells

    图  6  不同诱导子对三七悬浮细胞生物量及CGAs产量的影响

    Figure  6.  Effects of different elicitors on cells biomass and CGAs yield of P. notoginseng suspension cells

    图  7  三七细胞CGAs抗氧化能力评估及诱导前后HPLC图

    Figure  7.  Antioxidant activities of CGAs from P. notoginseng cells

    Vc is positive control; CK is CGAs from P. notoginseng cells without elicitor; MeJA is CGAs from P. notoginseng cells with 30 μM MeJA

    图  8  三七细胞CGAs与抗氧化活性的效应关系分析

    Figure  8.  Spectrum efficacy relationship between CGAs from P. notoginseng cells and antioxidant activity

    图  9  三七细胞CGAs对α-葡萄糖苷酶活性抑制

    Figure  9.  Inhibition of CGAs from P. notoginseng cells on α-glucosidase activity

    表  1  三七悬浮细胞生长及CGAs产物合成动力学参数

    Table  1.   Kinetics parameters for cells growth and CGAs biosynthesis by P. notoginseng suspension cells

    Medium systemGrowthCGAs biosynthesis
    μm/d−1R2α (mg CGAs/L)β (mg CGAs/L/d)R2
    Initial medium0.22±0.010.98−39.58±3.7610.60±0.480.99
    Optimized medium0.37±0.010.97−36.11±0.7212.06±0.180.99
    μm is the maximum specific growth rate; R2 is the correlation coefficient; α and β are model parameters
    下载: 导出CSV

    表  2  不同诱导子对三七悬浮细胞CGAs产量的影响

    Table  2.   Effects of different elicitors on CGAs yield in P. notoginseng suspension

    ElicitorsOptimum concentrationCGAs yield/(mg·L−1)Increase coefficient
    MeJA30 μmol/L684.071.44
    YE0.1 g/L598.191.26
    SA100 μmol/L518.571.09
    ANE0475.18No effect
    MT10 μmol/L517.701.09
    PDE25 mg/L622.071.31
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-02-23
  • 网络出版日期:  2022-05-19

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