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  • ISSN 1006-3080
  • CN 31-1691/TQ

小麦赤霉病新型复合天然抑菌剂的制备及其在小麦胚芽鞘上的验证

张晋东 白维祯 夏玮 张文清

张晋东, 白维祯, 夏玮, 张文清. 小麦赤霉病新型复合天然抑菌剂的制备及其在小麦胚芽鞘上的验证[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220124003
引用本文: 张晋东, 白维祯, 夏玮, 张文清. 小麦赤霉病新型复合天然抑菌剂的制备及其在小麦胚芽鞘上的验证[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20220124003
ZHANG jindong, BAI Weizhen, XIA Wei, ZHANG Wenqing. Preparation of A Novel Natural Compound Fungicide against Fusarium graminearum and its Verification on Wheat Coleoptiles[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220124003
Citation: ZHANG jindong, BAI Weizhen, XIA Wei, ZHANG Wenqing. Preparation of A Novel Natural Compound Fungicide against Fusarium graminearum and its Verification on Wheat Coleoptiles[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20220124003

小麦赤霉病新型复合天然抑菌剂的制备及其在小麦胚芽鞘上的验证

doi: 10.14135/j.cnki.1006-3080.20220124003
基金项目: 中国科学院重点资助项目
详细信息
    作者简介:

    张晋东(1996—),男,河北人,硕士生,研究方向为天然产物的应用,E-mail:ecust_zhjd@163.com

    通讯作者:

    张文清,E-mail:zhwqing@ecust.edu.cn

  • 中图分类号: O629.5

Preparation of A Novel Natural Compound Fungicide against Fusarium graminearum and its Verification on Wheat Coleoptiles

  • 摘要: 比较了肉桂醛、柠檬醛、香芹酚、芳樟醇、百里香酚、薄荷醇、紫苏醇对小麦赤霉病菌的体外抑菌效果,并进一步将香芹酚和百里香酚组合成新型复合天然抑菌剂。结果显示,当香芹酚和百里香酚质量比为1∶2时,抑菌剂对小麦赤霉病菌的体外抑制效果最佳,二者间具有协同作用。制备的抑菌剂能够改变病原菌细胞膜的通透性并显著抑制被侵染小麦胚芽鞘上病斑的生长。本文为开发具有精准功效的复合天然农药提供了理论指导和实用参考。

     

  • 图  1  复合天然抑菌剂配方筛选结果;小写字母a~d表示显著性差异(p<0.05).

    Figure  1.  Inhibitory effects on F.g. about different formulas; a~d means significant difference at p<0.05.

    图  2  上清液电导率值的变化;数据点附近的字母表示显著性差异(p<0.05),其中大写字母表示同时间不同浓度之间的差异,小写字母表示同浓度不同时间之间的差异;C.K.表示空白对照组。

    Figure  2.  Changes of conductivity of supernatant due to natural compound fungicides. Letters near each data indicate significant differences at p<0.05, where uppercase letters indicate differences between concentrations at the same time, lowercase letters indicate the difference between times at the same concentration. C.K. means blank control.

    图  3  新型复合天然抑菌剂对侵染小麦胚芽鞘的保护效果(A),治疗效果(B)及统计性分析(C);小写字母a~d表示显著性差异(p<0.05);C.K.表示空白对照组。

    Figure  3.  Protective (A), curative activity (B) and statistical analysis (C) of natural compound fungicide against F.g. on wheat coleoptiles. a~d means significant difference at p<0.05. C.K. means blank control.

    表  1  七种植物精油活性成分对F.g.的体外相对抑菌率 (%)

    Table  1.   Inhibitory effects of 7 active components from plant essential oils against F.g. (%) in vitro

    CinnamaldehydeCitralCarvacrolLinaloolThymolMentholPerillyl Alcohol
    R95.3±1.498.3±0.5100.0±0.062.3±2.9100.0±0.0100.0±0.094.7±0.2
    下载: 导出CSV

    表  2  不同浓度植物精油活性成分对F.g.的抑制效果

    Table  2.   Inhibitory effects of active components from plant essential oils at different concentrations

    ComponentEquationrEC50/(μg·mL−1)95% C.I./(μg·mL−1)
    Cinnamaldehydey=0.006x−0.9380.968166.794.8~273.3
    Citraly=0.007x−0.8510.988126.3107.8~145.5
    Carvacroly=0.016x−0.7310.99545.337.1~53.3
    Thymoly=0.018x−1.3020.98570.663.2~79.1
    Mentholy=0.010x−1.7900.999176.1160.7~194.3
    Perillyl Alcoholy=0.007x−1.3680.993189.5170.8~210.9
    *95 % C.I. indicates 95 % Confidence Interval
    下载: 导出CSV

    表  3  复合天然抑菌剂配方优化及作用特征

    Table  3.   Natural compound fungicide formula optimization results and S.I. for F.g.

    m(carvacrol)∶
    m(thymol)
    EquationrMmMtS.I.
    EC5095 % C.I.
    3∶1y=0.029x−1.6730.99657.152.2~62.949.80.87
    2∶1y=0.018x−0.9900.99754.847.9~63.151.40.94
    1∶1y=0.024x−1.2480.98652.246.7~58.555.21.06
    1∶2y=0.021x−0.8810.99341.235.6~47.459.61.45
    1∶3y=0.021x−0.9070.99742.436.8~48.662.01.46
    *95 % C.I. indicates 95 % Confidence Interval;The unit of Mm & Mt
    are μg·mL−1
    下载: 导出CSV

    表  4  新型复合天然抑菌剂对侵染小麦胚芽鞘上病斑长度的控制率

    Table  4.   Lesion length and its control rate of natural compound fungicide against F.g. on wheat coleoptiles

    Fungicide Concentration/
    (μg·mL−1)
    Protective ActivityCurative Activity
    Lesion Length/(cm)Control Rate/(%)Lesion Length/(cm)Control Rate/(%)
    50 0.83±0.09 a 42.84 c 0.79±0.09 a 45.77 c
    100 0.60±0.04 b 58.69 b 0.56±0.07 b 61.58 b
    200 0.30±0.06 c 79.08 a 0.23±0.08 c 84.54 a
    *Lowercase letters in each column indicate significant differences at p<0.05.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-01-24
  • 网络出版日期:  2022-05-07

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