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  • ISSN 1006-3080
  • CN 31-1691/TQ

肝细胞生长因子对人脐带间充质干细胞体外增殖的影响

王慧 刘佳星 周燕 谭文松

王慧, 刘佳星, 周燕, 谭文松. 肝细胞生长因子对人脐带间充质干细胞体外增殖的影响[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20210717001
引用本文: 王慧, 刘佳星, 周燕, 谭文松. 肝细胞生长因子对人脐带间充质干细胞体外增殖的影响[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-3080.20210717001
WANG Hui, LIU Jia-xing, ZHOU Yan, TAN Wen-song. Effect of Hepatocyte Growth Factor on Proliferation of Human Mesenchymal Stem Cells[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20210717001
Citation: WANG Hui, LIU Jia-xing, ZHOU Yan, TAN Wen-song. Effect of Hepatocyte Growth Factor on Proliferation of Human Mesenchymal Stem Cells[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-3080.20210717001

肝细胞生长因子对人脐带间充质干细胞体外增殖的影响

doi: 10.14135/j.cnki.1006-3080.20210717001
基金项目: 国家重点研发计划项目“生物医用材料研发与组织器官修复替代”(2018YFC1105800)。
详细信息
    作者简介:

    王慧:王 慧(1995—),女,山东菏泽人,硕士生,主要研究方向:细胞生物学。E-mail:whitneywang6071@163.com

    通讯作者:

    周 燕, E-mail:yanzhou@ecust.edu.cn

  • 中图分类号: Q291

Effect of Hepatocyte Growth Factor on Proliferation of Human Mesenchymal Stem Cells

  • 摘要: 为了改善无血清培养基促细胞增殖能力,探讨了肝细胞生长因子(Hepatocyte growth factor, HGF)对间充质干细胞(Mesenchymal stem cells, MSCs)体外增殖的影响。采用人脐带来源的MSCs,在实验室前期自制的无血清培养基SFM-O中添加不同浓度的HGF,采用CCK-8法测定细胞增殖;使用流式细胞术检测细胞周期,通过Western blot法检测细胞周期蛋白Cyclin D1及相关信号通路蛋白的表达。结果表明,在自制的无血清培养基中添加10 ng/mL的HGF能够有效促进MSCs的增殖。HGF能增强MSCs的整合素表达,进一步通过激活FAK-AKT-mTOR信号通路促进Cyclin D1蛋白合成,加速细胞周期从G1期到S期的转变,从而促进MSCs增殖。

     

  • 图  1  HGF对MSCs贴壁和增殖的影响

    Figure  1.  Effect of HGF on adhesion and proliferation of MSCs

    图  2  HGF对MSCs细胞周期的影响

    Figure  2.  Effect of HGF on cell cycle of MSCs

    图  3  HGF促进MSCs增殖的机制

    Figure  3.  Promotion mechanism of HGF on proliferation of MSCs

    (a~b) The effect of HGF on related protein in AKT pathway; (c~e) The effect of PF on signaling pathway and cell proliferation; (f~g) The effect of LY on signaling pathway and cell proliferation

    图  4  经SFM-O和SFM-H培养后MSCs的分化评价

    Figure  4.  Evaluation of differentiation of MSCs in SFM-O and SFM-H culture

    表  1  SFM-O及SFM-H组MSCs的表面表型表达率

    Table  1.   Expression rate of surface marker of MSCs in SFM-O and SFM-H

    SFM-OSFM-H
    Cell phenotypeExpression rate/%Cell phenotypeExpression rate/%
    CD4498.5CD4497.1
    CD7399.4CD7399.6
    CD9099.6CD9097.8
    CD10590.3CD10590.3
    CD140.388CD140.277
    CD340.054CD340.083
    CD450.053CD450.221
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出版历程
  • 收稿日期:  2021-07-17
  • 网络出版日期:  2022-09-20

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