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  • ISSN 1006-3080
  • CN 31-1691/TQ

基于茶树油/盐酸奥替尼啶的纳米胶囊的制备及抗菌性能

邢幸 康宁 曾天 徐云龙

邢幸, 康宁, 曾天, 徐云龙. 基于茶树油/盐酸奥替尼啶的纳米胶囊的制备及抗菌性能[J]. 华东理工大学学报(自然科学版), 2020, 46(5): 621-630. doi: 10.14135/j.cnki.1006-3080.20190625002
引用本文: 邢幸, 康宁, 曾天, 徐云龙. 基于茶树油/盐酸奥替尼啶的纳米胶囊的制备及抗菌性能[J]. 华东理工大学学报(自然科学版), 2020, 46(5): 621-630. doi: 10.14135/j.cnki.1006-3080.20190625002
XING Xing, KANG Ning, ZENG Tian, XU Yunlong. Preparation and Antibacterial Properties of Nanocapsules Based on Tea Tree Oil/Octenidine·2HCl[J]. Journal of East China University of Science and Technology, 2020, 46(5): 621-630. doi: 10.14135/j.cnki.1006-3080.20190625002
Citation: XING Xing, KANG Ning, ZENG Tian, XU Yunlong. Preparation and Antibacterial Properties of Nanocapsules Based on Tea Tree Oil/Octenidine·2HCl[J]. Journal of East China University of Science and Technology, 2020, 46(5): 621-630. doi: 10.14135/j.cnki.1006-3080.20190625002

基于茶树油/盐酸奥替尼啶的纳米胶囊的制备及抗菌性能

doi: 10.14135/j.cnki.1006-3080.20190625002
基金项目: 上海市纳米技术专项基金(编号11nm0500900)、上海市学科带头人(编号B502)和上海市重点实验室(编号08DZ2230500)资助
详细信息
    作者简介:

    邢幸:邢 幸(1989-),男,山东威海人,硕士生,主要从事生物医用材料的研究。E-mail:13601734659@163.com

    通讯作者:

    徐云龙,E-mail:xuyunlong@ecust.edu.cn

  • 中图分类号: R751.05

Preparation and Antibacterial Properties of Nanocapsules Based on Tea Tree Oil/Octenidine·2HCl

  • 摘要: 以双吡啶胺类广谱抗菌药盐酸奥替尼啶(OCT, Octenidine·2HCl)为乳化剂,正硅酸乙酯(TEOS)为硅源,用微乳液法成功制备出包裹茶树油(TTO)的纳米二氧化硅胶囊SINC-OCT/TTO。随后仅将乳化剂更换为十六烷基三甲基氯化铵(CTAC),以同样的方法和配比制备包裹茶树油的纳米二氧化硅胶囊SINC-TTO。通过扫描电镜、透射电镜、粒度分析、红外光谱、热重分析、氮气吸附-脱附等表征方法对SINC-OCT/TTO 和 SINC-TTO这两种纳米胶囊(SINCs)进行形貌和结构表征,并进行抗菌性能和释药性能测试。结果表明:两种纳米胶囊呈规整的空心球形结构并且具有良好的药物缓释性能。纳米胶囊的结构受乳化剂的种类影响,SINC-OCT/TTO的流体力学直径、比表面积、包药量均大于SINC-TTO。联合药敏实验分析结果表明,TTO与OCT联用可以对金黄色葡萄球菌起到协同抗菌作用;对大肠杆菌起到相加抗菌作用。纳米胶囊的抗菌实验结果证实SINC-OCT/TTO的杀菌性能远高于SINC-TTO。

     

  • 图  1  微乳液法合成二氧化硅纳米胶囊的原理图

    Figure  1.  Schematic illustration of the synthesis of silica nanocapsules(SINCs) by using miniemulsion

    图  2  SINC-TTO,SINC-OCT/TTO粒径分布曲线

    Figure  2.  Particle size distribution of SINC-TTO and SINC-OCT/ TTO     

    图  3  SINC-TTO,SINC-OCT/TTO,SINC-OCT纳米胶囊的扫描电镜与透射电镜照片

    Figure  3.  SEM and TEM micrographs of the SINC-TTO, SINC-OCT/TTO and SINC-OCT

    图  4  SINC-TTO,SINC-OCT/TTO的氮气吸附-脱附曲线

    Figure  4.  N2 adsorption-desorption isotherms of SINC-TTO and SINC-OCT/TTO

    图  5  (a) 两种载药纳米胶囊冻干粉末的热重分析曲线;(b) 茶树油、二氧化硅及两种载药胶囊红外光谱图

    Figure  5.  (a) TGA curves of freeze dried SINCs; (b) FT-IR spectra of TTO, SiO2, two kinds of SINCs

    图  6  两种药物单独作用 (a, b, c, d) 与联合作用 (e, f) 时药物浓度与细菌存活率的关系

    Figure  6.  Relationship between the two kinds of drug alone (a, b, c, d) and combined (e, f) act on the bacterial concentration and the survival rate of bacterial

    图  7  (a) 茶树油乙醇溶液和 (b) 盐酸奥替尼啶水溶液的标准曲线; 两种纳米胶囊释放的 (c) 茶树油和 (d) 盐酸奥替尼啶质量浓度随时间的变化曲线

    Figure  7.  Calibration curves for the determination of TTO in ethyl alcohol (a) and OCT in water (b); Release of (c) TTO and (d) OCT from SINCs

    图  8  不同SINCs对金黄色葡萄球菌(a)和大肠杆菌(b)的灭活效率

    Figure  8.  Inactivation efficiency of S.aureus (a) and E. coli (b) in the presence of different SINCs

    表  1  两种用不同表面活性剂合成的包裹茶树油纳米胶囊的流体力学直径、包油率、比表面积、孔径

    Table  1.   Hydrodynamic diameters, encapsulation efficiency, surface area and mean pore diameter of silica nanocapsules containing tea tree oil stabilized by different surfactants

    SampleOilSurfactantdn/nmMass fraction of TTO in the capsules /%Surface area/ (m2·g−1)Mean pore diameter /nm
    SINC-TTOTTOCTAC67.3633.328910
    SINC-OCT/TTOTTOOCT121.747.74375.6
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出版历程
  • 收稿日期:  2019-06-25
  • 网络出版日期:  2020-07-15
  • 刊出日期:  2020-10-30

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