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    薛佳韵, 赵明霞, 王泽建, 孙慧婕, 王永红, 储炬, 庄英萍. 豆油添加对尼莫克汀发酵的影响[J]. 华东理工大学学报(自然科学版), 2020, 46(4): 533-542. DOI: 10.14135/j.cnki.1006-3080.20190428002
    引用本文: 薛佳韵, 赵明霞, 王泽建, 孙慧婕, 王永红, 储炬, 庄英萍. 豆油添加对尼莫克汀发酵的影响[J]. 华东理工大学学报(自然科学版), 2020, 46(4): 533-542. DOI: 10.14135/j.cnki.1006-3080.20190428002
    XUE Jiayun, ZHAO Mingxia, WANG Zejian, SUN Huijie, WANG Yonghong, CHU Ju, ZHUANG Yingping. Effects of Soybean Oil on Streptomyces Cyaneogriseus ssp. Noncyanogenus Fermentation[J]. Journal of East China University of Science and Technology, 2020, 46(4): 533-542. DOI: 10.14135/j.cnki.1006-3080.20190428002
    Citation: XUE Jiayun, ZHAO Mingxia, WANG Zejian, SUN Huijie, WANG Yonghong, CHU Ju, ZHUANG Yingping. Effects of Soybean Oil on Streptomyces Cyaneogriseus ssp. Noncyanogenus Fermentation[J]. Journal of East China University of Science and Technology, 2020, 46(4): 533-542. DOI: 10.14135/j.cnki.1006-3080.20190428002

    豆油添加对尼莫克汀发酵的影响

    Effects of Soybean Oil on Streptomyces Cyaneogriseus ssp. Noncyanogenus Fermentation

    • 摘要: 考察了发酵过程中豆油对尼莫克汀合成的影响,并建立了优化的豆油补加策略。研究结果表明,在5 L反应器中初始添加1.5 g/L豆油后,尼莫克汀的生物量有明显提高,与对照组相比,干重提高了29%,尼莫克汀的效价提高了27.37%,产量达到1 475 μg/mL,结合发酵相关参数分析,推测初始添加豆油能够有效提高蓝灰色链霉菌的初级代谢活性,并能有效提高尼莫克汀的合成速率以及延长尼莫克汀的高速合成期,从而显著提升尼莫克汀的发酵产量。

       

      Abstract: Nemadectin produced by Streptomyces cyaneogriseus ssp. noncyanogenus ( S. cyaneogriseus ) is a 16-membered macrocyclic lactones antibiotics, which belongs to milbemycin subfamily. Moxidectin is chemically synthesized from nemadectin, which has broad application prospects on agricultural insecticides field because of its high efficiency, safety, environmentally friendly and so on. Therefore, many researches have paid more attention in the past few decades and most of the researches were focused on genomic, chemical modification and pharmacological aspects, such as the global genome sequence ananlysis of S. cyaneogriseus, and toxicology of moxidectin on the animal deworming. However, any researches hardly focused on the fermentation technology of process optimization and regulation of nemadectin fermentation are scarce up to now. In this study, soybean oil had a positive effect nemadetin fermentation. The biomass of S. cyaneogriseus was effectively improved by adding soybean oil at start in 5 L fermentation (for about 29%), and the titer of nemadectin rised up to 1 475 μg/mL, which was increased by 27.37%. Based on the analysis of the fermentation parameters, The primary metabolic activity of S. cyaneogriseus and the synthesis rate of nemadectin was greatly improved by the addition of soybean oil at the start of fermentation, resulting in a significant increase in the total yield of nemadectin.

       

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