On line Determination of OUR,CER and RQ in Shake Flask

A shake flask was designed and prepared specially for the on line determination of oxygen,nitrogen and carbon dioxide concentrations in the outlet gas.So,oxygen uptake rate ( OUR ),carbon dioxide evolution rate ( CER ) and respiration quotient (RQ) were calculated by this method successfully.It shows that the apparatus and the results of above mentioned parameters measurement are relible in the flask fermentation of baker's yeast.