Cloning and Characterization of an Endoglucanase from Bacillus sp. ECU0013

Based on a putative endoglucanase gene of Bacillus subtilis, we designed the primers and cloned a recombinant EG gene from Bacillus sp. ECU0013. Enzyme BsEG was successfully overexpressed in Escherichia coli and purified to homogeneity by Ni-NTA column. The maximal activity could be reached when pH was about 6.0 and reaction temperature was 50 ℃. The half-life time of EGbs at 50 ℃ was up to 101 h. K_m and V_max were 20.1 g/L and 0.075　g/(L·min), respectively. Futhermore, the substrate specificity and cellulose absorption behavior of BsEG were studied.