Effect of Butyrate on Cell Growth and Expression of EPO in Recombinant CHO Cells

Recombinant CHO cells were cultured in CHO\|S\|SFM II.The effects of sodium butyrate on cell growth,glucose metabolism and EPO expression were investigated.Cell growth decreased with the addition of sodium butyrate in the serum\|free medium and was entirely inhibited at 2 mmol/L sodium butyrate. The specific rate of glucose utilization was also reduced by 30% in the presence of sodium butyrate.In addition,EPO expression was not facilitated by the addition of sodium butyrate,but its addition may be helpful to maintain the stability of EPO productivity.