Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
Citation:
Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
Citation:
Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum
Methyluridine was enzymatically produced from guanosine and thymine by intact cells of Brevibacterium acetylicum QD9805. The optimal reaction conditions were researched. When the reaction mixtures, containing 100200mmol/L substrates, 1050mmol/L phosphate buffer(pH 8.0),were incubated at 60C shaking at 120r/min for 1h, the conversion yield could reach above 60%.
DownLoad: