High Cell-Density Culture of E.coli TB1/pMAL-hOCIFm

Batch and fed-batch culture process of E.coli TB1/pMAL-hOCIFm were carried out to produce recombinant human osteoclastogenesis inhibitory factor mature peptide (rhOCIFm) in 3.7 L Bioengineering autocontrol fermentor. High cell-density and high expression were achieved by the control of carbon/nitrogen source and dissolved oxygen. The final cell density and concentration of rhOCIFm-MBP (fusion) protein were 57.8 OD_(600) and 3.2 g/L respectively. The purity of rhOCIFm-MBP fusin protein was more than 70% after treated with affinity chromatography on amylose resin. This study provides a basic work for the production of rhOCIFm in industrial scale.