Soluble Expression of S-Adenosylmethionine Synthetase

This paper systematically investigated the expression of Sadenosylmethionine synthetase gene from yeast by using the pET soluble expression system. It was indicated that pET44a (Nus fusion tag ) as the carrier and Origami (trxB and gor double mutant) as the host were suitable for the soluble expression of the target protein. Furthermore, the same result was obtained when the soluble expression of Sadenosylmethionine synthetase gene from different sources (E. coli, Bacillus subtilis, Bacillus thuringiensiss) were tested. It was also found that the gene from yeast got the highest soluble expression, which the specific activity of SAM synthetase was up to 60.9 U/mg.