A Preliminary Test on the Effect of Oxygen Supplyon the Growth and Anthocyanin Production by Cell Cultures of Perilla frutescens in Bioreactors

Effect of oxygen supply on the suspended cultures of Perilla fruteseens plantcells was demonstrated in various cultivation vessels.In flask cultures, Produetion of antho-cyanin pigment was increased with improvement of oxygen supply conditions.In cultivationsusing a 2L(working volume)bubble columm reactor and a 3L stirred reactor with a marineimpeller,the metabolite produetion was also conspieuously inereased by improving the condition of oxygen supply. However,it was observed that too strong aeration was harmful to thecell cultures,which may be due to high shear stress under the condition.