Influence of Nitrogen Sources on Synthesis and Secretion of Gentamicin

The effects of inorganic and organic nitrogen sources on synthesis and secretion of gentamicin in shake flask fermetation were studied. (NH 4) 2SO 4,NaNO 3 and various amino acids were added initially and in biosynthesis phase. The results showed that total potency increased by 23% when the concentration of (NH 4) 2SO 4 was 0.1g/L; component of gentamicin was also influenced by (NH 4) 2SO 4; total potency incresased by 55% when 8g/L NaNO 3 was added at 24h; secretion ratio increased by 33% when 8g/L NaNO 3 was added intially; total potency increased by 64% when 0.8g/L lysine was added in the biosynthesis phase. At the same time, verified experiment was done in the resting cell system.