Immobilization of Antitumor Agent L asparaginase on Chitin

The optimum immobilization conditions and some properties of immobilized L asparaginase with chitin as the support were studied. The dosage of crosslinking agent, buffer pH value and activation time of carrier played some importat roles in L asparaginase immobilization. The maximum relative activity of immobilized L asparaginase could reach 25.4%. The researches on properties of immobilized L asparaginase demonstrated that the resistance to pepsin hydrolysis and storage stability of immobilized L asparaginase were increased greatly compared with that of free enzyme.