Preparation of Flurbiprofen Axetil Lipid Microspheres and Their Pharmacokinetics in Rabbits

Flurbiprofen axetil (FA) loaded lipid microspheres (LM) were prepared by two step emulsification method. Their mean size was (168.6 ± 9.3) nm as determined by dynamic light scattering and ultracentrifugation. The zeta potential was (-34.8 ± 1.4) mV, and entrapment efficiency was (99.4±0.9)%. A high performance liquid chromatography (HPLC) method was established to detect flurbiprofen (FP) concentration of the FA loaded LM in rabbits after intravenous injection. Pharmacokinetic parameters were t1/2β: (0.83 ± 0.13) h, AUC0→24 h:( 16.10 ± 1.56) μg·h/mL, CL: (0.41 ± 0.02) L/（h·kg), respectively. The obtained LM showed high entrapment efficiency of FA with high stability. The plasma concentration of FP showed that the pharmacokinetics of FA loaded LM was well fitted by two compartments model.