Cloning and Identification of Alginate Lyases Genes from Vibrio alginolyticus ATCC 17749

Finding new sources of alginate lyase gene had a certain scientific research significance and potential application value. In this study, five putative alginate lyase genes were cloned from Vibrio alginolyticus ATCC 17749 according to methods of bioinformatics. Heterologous expressions of five genes in Escherichia coli were achieved by constructing five expression plasmids pET28a algV1，pET28a algV2，pET28a algV3，pET28a algV4，pET28a algV5. Products coded by five genes were verified to own alginate lyase activity by qualitative and quantitative determination. In addition, alginate lyases genes algV1, algV2 and algV3 were extracellular enzyme, and algV4 and algV5 were intracellular enzyme.