Cloning and Expression of TATapoptin and Its Activity Study in vitro

A basic peptide derived from human immunodeficiency virus HIV1 TAT peptide (positions 47-57) can translocate through the cell membranes, the characteristics of which are utilized for the delivery of exogenous proteins into cells. The coding sequence for the TATapoptin was amplified by polymerase chain reaction(PCR)， then inserted into pET28b vector and highly expressed in Escherichia coli BL21(DE3). The protein expressed in inclusion body was purified by NiNTA under denaturing condition. Apoptosis activity of the TAT apoptin was evaluated by MTT. Results indicated that HeLa cells were highly sensitive to the purified TAT apoptin.