Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase

A novel esterase which stereoselectively hydrolyzes the( )-trans stereoisomer of ethyl chrysanthemate to produce( )-trans-chrysanthemic acid was discussed.The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction(PCR).The gene was inserted into a vector and expressed in Escherichia coli BL21(DE3).The protein expressed in body was dissolve in 8 mol/L urea before dialysis.The purified crude enzyme show high catalysis activity.It may suggest the possibility that( )-trans-chrysanthemate is identified as the insecticide.