Genome Re annotation, Novel Strong Promoter Discovery and Sequence Analysis of Pichia Pastoris Based on RNA-Seq

The transcriptome of Pichia pastoris was sequenced using RNA Seq technique. Based on the sequencing data, the genome of Pichia pastoris was re annotated, including 861 revised positions of the genome sequence, 553 re annotated transcripts, 249 newly discovered transcripts and 83 alternative splices. In the analysis of gene expression profiling, 2 novel strong promoters, named P437 and P1431 respectively, were discovered. The highest transcription levels of the transcripts directed by these promoters are, respectively, 1.78 times and 3.40 times of that of AOX1 gene. Several binding sites of transcription factors were revealed in P437 and P1431 by sequence analysis.