Synthesis and Purification of Unsymmetrical Water-soluble Cyanine Dyes

Two water soluble unsymmetrical cyanine dyes, which can be used as fluorescence tag in DNA labeling, was synthesized from p hydrazino benzenesulfonic acid by 6 steps. A ternary solvent were developed and used as eluant in purification of intermediates and final dyes by PTLC method. The yields were as good as that of the HPLC method that had been reported.