Fluorometric assay of fluorescence-labeled proteins in the polyacrylamide gel electrophoresis systems

The isolation of fluorescamine or p-chlorofluorescamine-labeled proteins in the po-lyacrylamide gel electrophoresis systems can be carried out at room temperature. A pro-tein sample can be isolated and its discs identified within two hours and a half, time thusspent being only a quarter as much as that of the traditional method and identification ofsharp and well-defined protein discs made possible with a much higher sensitivity.