Genetic Manipulation of the Genes Related with Shikimate Metabolism of Escherichia coli

In order to investigate how the genetic manipulation with related genes in Escherichia coli impacts the shikimate metabolism and the accumulation of shikimic acid, shikimate kinase II gene (aroL) of Escherichia coli JM83　was disrupted using temperature sensitive plasmid and the mutant was named as JDL02. Meanwhile, ppsA, tktA and aroG gene were cloned from Escherichia coli JM83. A series of expression plasmids were constructed and transferred into JDL02. Flask fermentation verified that the yields of all the recombinant strains are improved. In the same bacterial density condition, the shikimic acid yield of JDL02/pTrc-aroG-tktA strain was 18.35 times of the control JM83, and the flask fermentation yield is 94.33 mg/L.