Investigation on Purification of Major Allergen in Platanus Hispanica Pollen
-
Graphical Abstract
-
Abstract
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography. The bioactivity was identified by BSA ELISA which showed the highest allergenic activity. Their molecular weights determined by SDS PAGE were 20 ku and 18 ku respectively.
-
-