Optimization of Medium Components for Adenosine Production by Bacillus subtilis Using Response Surface Design

Response surface design was used to optimize the medium components for adenosine production by Bacillus subtils. Firstly, effects of yeast extract, corn steep liquor, KH_2PO_4, MgSO_4, (NH_4)_2SO_4 on adenosine production were evaluated using a 2-level factorial design. Among the components investigated, yeast extract, KH_2PO_4 and (NH_4)_2SO_4 played important roles in adenosine production, while others had little effect. Then a central composite design was used to optimize the significant factors and the (results) were shown in response surface plots. When the medium comprised (g/L) yeast extract 15, KH_2PO_4 6 and (NH_4)_2SO_4 10, the adenosine yield was 7.42 g/L, which was 28.6% higher than the (control.)