Study on the Immobilization of L -asparaginase with Ethylenediamine-hydroxypropyl Chitosan

A novel aminated chitosan derivative, ethylenediamine hydroxypropyl chitosan (EDA HPCS), was prepared and was applied to immobilize L asparaginase. The effects of formadehyde activated and glutaraldehyde activated carriers on the activities of immobilized enzyme were investigated. The results showed that, at proper immobilization conditions, the activities of immobilized enzyme with formadehyde activated EDA HPCS were about 30 U/g and the activities of immobilized enzyme with glutaraldehyde activated EDA HPCS were about 16U/g.