Effect of Substrate Induction and Fed-batch Operation on Biosynthesis of Epoxide Hydrolase from Bacillus megaterium ECU1001

An epoxide hydrolase biosynthesized by Bacillus megaterium EUC1001 isolated from soil can hydrolyze racemic phenyl glycidyl ether (PGE) with high enantioselectivity. Initial activities of the epoxide hydrolase obtained in flask and 5L fermentor were 11.0U/L and 31.0U/L, respectively. The enzyme activity in flask reached 61.0U/L by adding the substrate as an inducer and it could even reach 96.6U/L through fed batch fermentation in a 5L fermentor.