Preparation of pHSensitive Doxorubicin Liposomes Modified with Carboxymethyl Chitosan and in Vitro Cell Research

To enhance the accumulation in the tumor and intracellular delivery of the encapsulated doxorubicin(DOX), a pHsensitive doxorubicin nanoliposomes(DOXNL) modified with carboxymethyl chitosan(CMCT) was prepared by thinfilm pH gradient. The nanoliposomes modified with CMCT showed good shape with an average particle size of (38±221) nm and the drug encapsulation efficiency was about 8883%. The drug release of the CMCTmodified DOX nanoliposomes in vitro showed obvious pH sensitivity. The Hela cells treated with CMCTDOXNL showed remarkable increase in cellular association or DOX uptake compared with DOXNL. The cytotoxicity of CMCTDOXNL on Hela cells was higher than that of DOXNL. The pHsensitive CMCTmodified DOX nanoliposomes promoted the delivery of the encapsulated DOX into the tumor cells.