Construction of Small-Sized Blood Vessel Using SCs in vitro

A set of bioreactor system was designed with the application of finite element methods as an analysis method to construct tissue engineered blood vessels in the diameter of 2 mm. hBMSCs and ADSCs were first collected and further cultivated. The third passage of the cultivated cells were then seeded on the poly(glycolic acid) (PGA) to fabricate the cellscaffold compound. Subsequently, this compound was subjected to dynamical culturing in the blood vessel bioreactor. Tissueengineered blood vessels can be constructed by culturing the hBMSCsPGA and hADSCs-PGA compound in a dynamic condition. After cultered for 4 weeks, the tissues were removed from the bioreactor and subjected to analysis by gross view, HE and SEM(scanning electron microscope). It was observed that the tissueengineered blood vessel has a bright color and certain elasticity. The secreted collagen matrix arrayed orderly around the cells and smooth muscle elastic actin can also be detected in the formed tissues. In conclusion, biological conditions of blood vessels can be simulated using the current blood vessel bioreactor system. Using hBMSCs and ADSCs, the construction of the tissue engineered smallsized blood vessels can be successfully achieved in our bioreactor system.