Apoptin Fusion Protein Transduced in HeLa Cells and Its Activity Assay in vitro

To study transduction activity of the heparinbinding domain（HBD）of extracellular superoxide dismutase （EC-SOD）in HeLa cell, and to confirm HBD have the ability to deliver pharmaceutical proteins (apoptin) into cells and to induce apoptosis of tumor cells. Cloned HBD-EGFP fusion protein was expressed and purified by Ni2+- NTA affinity chromatography, to observe its transduction activity. HBD-apoptin fusion protein was expressed and purified, then the apoptosis of tumor cells in vitro was tested by MTT assay. The results show that green fluorescence can be observed in HeLa cells using fluorescence microscopy. MTT results indicated that fusion protein of apoptin can induce apoptosis of HeLa cells. HBD with the transduction activity, was capable of carrying drugs protein (apoptin) to enter cells and can induce apoptosis in HeLa cells.