Cloning and Expression of Bacillus subtilis ATCC21216 Adenosine Phosphorylase Gene in Escherichia coli

The gene deoD(0.7 kb) of B.subtilis ATCC21216 encoding an adenosine phosphorylase was amplified by polymerase chain reaction(PCR).Results of sequencing showed 98.7% similarity to the same gene of B.subtilis 168.Based on DNA recombinant techniques,the gene deoD was cloned into plasmid pET28a( )(5.3 kb) to generate highly effective E.coli BL21(DE3) strain producing adenosine phosphorylase.Expression of the recombinant protein was 27% of the total protein.Specific activity of the(enzyme) in crude extract determined by high performance liquid chromatography was 0.151 U/mg protein.