Influence of Metabolic Byproducts on Growth and Metabolism of Hybridoma Cells in Serum-free CulturesI. Effect of Lactate

In the batch cultures of WuT3 hybridoma cells in serum free medium with different lactate concentrations, the influences of lactate on cells growth and metabolism were investigated. It was found that lactate below 5.3mmol/L influenced hardly on cells, lactate between 10mmol/L and 25mmol/L inhibited a little on cells growth and metabolism, and lactate above 34mmol/L inhibited strongly on cell cultures.