Cloning and Expression of Vitreoscilla Hemoglobin in Escherichia coli for TRAIL Production

Vitreoscilla hemoglobin gene(vgb) was cloned and expressed in Escherichia coli C600(pBV220-TRAIL) for improving the oxygen uptake rate(OUR) and cell density.PCR reactions and carbon monoxide different spectra analysis showed that vgb was successfully expressed in C600(pBV220-TRAIL),and this expression system was regulated by dissolved oxygen(DO).Recombinant engineering strain CTV maintained genetic stability well.Under the same culture conditions,the highest cell density of CTV(50.6 g/L) and the yield of TRAIL(2.8 g/L)was 1.64-fold and 1.7-fold higher than that of control in 3.7 L bioreactor,respectively,whereas the acetic acid concentration was decreased by 45%.