Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells

In this paper, Panax notoginseng (P. notoginseng) suspension cells were used for the production of chlorogenic acids (CGAs), of which bioactivities were evaluated. Firstly, CGAs in P. notoginseng suspension cells were identified by liquid chromatography-tandem mass spectrometry. Secondly, the suspension culture system and elicitation mode were optimized. Ultimately, the antioxidant and α-glucosidase inhibitory activity of CGAs from P. notoginseng cells were determined. Four CGAs were inferred inP. notoginseng cells. The optimal condition was obtained (B5+40 g/L sucrose, pH 7.5 and 15% inoculation size). By co-culture with 30 μmol/L methyl jasmonate for 3 d, the CGAs yield could reach up to 684.07 mg/L, which was 1.44 times that of the control. CGAs from P. notoginseng cells showed an excellent antioxidant capacity in radical-scavenging test and, moreover, certain inhibition on α-glucosidase activity. Our results indicate that the production of CGAs by P. notoginseng cells has potential applications in healthy food and daily cosmetics.