Abstract:
In this paper,
Panax notoginseng (
P. notoginseng) suspension cells were used for the production of chlorogenic acids (CGAs), of which bioactivities were evaluated. Firstly, CGAs in
P. notoginseng suspension cells were identified by liquid chromatography-tandem mass spectrometry. Secondly, the suspension culture system and elicitation mode were optimized. Ultimately, the antioxidant and
α-glucosidase inhibitory activity of CGAs from
P. notoginseng cells were determined. Four CGAs were inferred in
P. notoginseng cells. The optimal condition was obtained (B5+40 g/L sucrose, pH 7.5 and 15% inoculation size). By co-culture with 30 μmol/L methyl jasmonate for 3 d, the CGAs yield could reach up to 684.07 mg/L, which was 1.44 times that of the control. CGAs from
P. notoginseng cells showed an excellent antioxidant capacity in radical-scavenging test and, moreover, certain inhibition on
α-glucosidase activity. Our results indicate that the production of CGAs by
P. notoginseng cells has potential applications in healthy food and daily cosmetics.