Abstract: Smart nano-pesticides have been prepared to efficiently deliver a sufficient amount of active ingredients in response to different stimuli, employing targeted and controlled release mechanisms. In this paper, an enzyme-responsive carrier for controlled release of lambda-cyhalothrin (LC) was prepared through loading LC into aminated hollow mesoporous silica (HMSN) nanoparticles by physical adsorption, and then carboxymethyl-β-cyclodextrin (CM-β-CD) was coated onto the surface of HMSN using amidation reaction as the blocking molecule. A series of physicochemical characterizations showed the successful construction of the CM-β-CD/LC/HMSN material. An in vitro release test showed that the LC release amount from CM-β-CD /LC/HMSN reached up to 55% in the presence of α-amylase, while that was only about 20% in the absence of the enzyme, suggesting that the pesticide release was dependent on enzymatic activity. Biological tests showed that the pesticide formulation had good insecticidal effect on Mythimna separata larvae. Based on the results obtained by different experimental methods, we believe that the nanoparticles would be useful for releasing LC after degradation of cyclodextrin enzymatically in vivo , suppressing the survival of Mythimna separata larvae.