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    刘靖康, 李猛, 王铭纬, 徐益升. 基于瞬时纳米沉淀法的球形纳米粒子电荷及粒径调控[J]. 华东理工大学学报(自然科学版), 2020, 46(3): 334-340. DOI: 10.14135/j.cnki.1006-3080.20190227003
    引用本文: 刘靖康, 李猛, 王铭纬, 徐益升. 基于瞬时纳米沉淀法的球形纳米粒子电荷及粒径调控[J]. 华东理工大学学报(自然科学版), 2020, 46(3): 334-340. DOI: 10.14135/j.cnki.1006-3080.20190227003
    LIU Jingkang, LI Meng, WANG Mingwei, XU Yisheng. Charge and Size Modulation of Spherical Nanoparticles Using Flash Nanoprecipitation[J]. Journal of East China University of Science and Technology, 2020, 46(3): 334-340. DOI: 10.14135/j.cnki.1006-3080.20190227003
    Citation: LIU Jingkang, LI Meng, WANG Mingwei, XU Yisheng. Charge and Size Modulation of Spherical Nanoparticles Using Flash Nanoprecipitation[J]. Journal of East China University of Science and Technology, 2020, 46(3): 334-340. DOI: 10.14135/j.cnki.1006-3080.20190227003

    基于瞬时纳米沉淀法的球形纳米粒子电荷及粒径调控

    Charge and Size Modulation of Spherical Nanoparticles Using Flash Nanoprecipitation

    • 摘要: 合成了阳离子型嵌段共聚物聚甲基丙烯酸-2-(二甲氨基)乙酯-b-聚己内酯(PDMAEMA-b-PCL),并以此嵌段共聚物与生物相容的嵌段共聚物聚氧乙烯-b-聚己内酯(PEG-b-PCL)的混合物为稳定剂,通过瞬时纳米沉淀法(FNP)制备包载疏水药物β-胡萝卜素的球形载药纳米粒子。实验结果表明,通过调节嵌段共聚物质量比和进料流速可以对纳米粒子的表面电荷和粒径进行调节,所制备的载药纳米粒子具有一定的pH响应性,并且在癌症诊断和治疗方面具有潜在的应用价值。

       

      Abstract: Size and surface charge of nanoparticles have important impacts on drug delivery process. Conventional preparation and tuning of particle features e.g. surface charge are difficult and time consuming. In this work, a pH-responsive spherical drug-loaded nanoparticles was prepared by encapsulating a hydrophobic drug β-carotene in a biocompatible copolymer, poly(ethylene glycol)-b-polycaprolactone (PEG-b-PCL) and poly(2-(dimethylamino) ethylmethylacrylate)-b-polycaprolactone (PDMAEMA-b-PCL), via flash nanoprecipitation (FNP) method in a multi-inlet vortex mixer (MIVM). Particle size and surface charge of nanoparticles at different pH values were investigated. By changing the feed ratio of the two polymers, surface charge of the nanoparticles can be tuned from +32.4 mV to −4.7 mV. Meanwhile, as the ratio of PEG-b-PCL was increased, the surface charge decreased due to the decrease of amine content from the PDMAEMA block. The size of the nanoparticles can be adjusted by changing the feed flow rate into the MIVM by which the flow rate of organic phase (v1) was kept at 12 mL/min and the flow rate of water (v2) was increased from 24 mL/min to 96 mL/min, the nanoparticles size was decreased from 126.6 nm to 62.7 nm. When v1: v2 was 6∶24, 12∶48, 24∶96, respectively, the nanoparticles size can be reduced from 107.5 nm to 77.3 nm. The nanoparticle is stable for 25 d at pH=7.4. By the strategy of flash nanoprecipitation, the spherical drug-loaded nanoparticles can be rapidly prepared, particle size and surface charge can be accurately modulated easily and provided potential applications for cancer diagnosis and treatments in the future.

       

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