高级检索

  • ISSN 1006-3080
  • CN 31-1691/TQ

喹啉腈荧光微球构建及在荧光免疫层析中对SAA的检测应用

李强 徐建新 郭志前

李强, 徐建新, 郭志前. 喹啉腈荧光微球构建及在荧光免疫层析中对SAA的检测应用[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-2080.20220414001
引用本文: 李强, 徐建新, 郭志前. 喹啉腈荧光微球构建及在荧光免疫层析中对SAA的检测应用[J]. 华东理工大学学报(自然科学版). doi: 10.14135/j.cnki.1006-2080.20220414001
LI Qiang, XU Jianxin, GUO Zhiqian. Construction of Quinoline-malononitrile Fluorescent Microspheres for the Detection of SAA via Fluorescent Immunochromatography[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-2080.20220414001
Citation: LI Qiang, XU Jianxin, GUO Zhiqian. Construction of Quinoline-malononitrile Fluorescent Microspheres for the Detection of SAA via Fluorescent Immunochromatography[J]. Journal of East China University of Science and Technology. doi: 10.14135/j.cnki.1006-2080.20220414001

喹啉腈荧光微球构建及在荧光免疫层析中对SAA的检测应用

doi: 10.14135/j.cnki.1006-2080.20220414001
基金项目: 国家自然科学基金面上项目(21878087 )
详细信息
    作者简介:

    李强(1990-),男,上海人,博士,主要从事功能性荧光染料微球的研究。E-mail: liq0311@163.com

    通讯作者:

    郭志前,E-mail:guozq@ecust.edu.cn

  • 中图分类号: O69

Construction of Quinoline-malononitrile Fluorescent Microspheres for the Detection of SAA via Fluorescent Immunochromatography

  • 摘要: 基于聚集诱导发光(AIE)喹啉腈(QM)染料的衍生物QM-OH制备了乳胶荧光微球QM-OH@PS-COOH(羧基聚苯乙烯),通过扫描电子显微镜等表征仪器研究了微球的形貌结构,并进一步将该荧光微球应用于荧光免疫层析法检测中,实现了对临床样本中血清淀粉样蛋白A(SAA)含量变化的灵敏检测。研究结果表明:QM-OH@PS-COOH荧光微球大小均一、形状规整,与市售荧光微球对比,其密度、固含量、羧基含量基本一致;该荧光微球体系不仅可以定性区分炎症感染,而且能对SAA含量进行定量分析,实现了对炎症感染的有效评估和监控。因此,基于QM-OH@PS-COOH的检测平台可用于早期疾病检测的筛查,特别是用于即时检测领域中的自我检测。

     

  • 图  1  激发波长440 nm下化合物QM-OH在水和四氢呋喃体系中(不同水质量分数)的紫外吸收图(a)和荧光光谱图(b)

    Figure  1.  Absorption (a) and emission (b) spectra of QM-OH in H2O/THF mixtures with different volume fraction of water (fw) under λ ex = 440 nm

    图  2  AIE荧光微球QM-OH@PS-COOH的TEM图,插图是QM-OH@PS-COOH的乳液图片

    Figure  2.  TEM image of QM-OH@PS-COOH, illustrated by emulsion image of QM-OH@PS-COOH

    图  3  AIE荧光微球QM-OH@PS-COOH的表面羧基电导滴定变化图

    Figure  3.  Surface carboxyl conductance titration of QM-OH@PS-COOH

    图  4  喹啉腈AIE荧光微球QM-OH@PS-COOH荧光免疫层析检测SAA原理示意图

    Figure  4.  Schematic diagram of quinoline-malononitrile AIE fluorescent microsphere QM-OH@PS-COOH for SAA detection in fluorescent immunochromato graphy

    图  5  SAA质控品定标的曲线

    Figure  5.  Calibration curve of SAA quality control product

    图  6  (a)MAYA荧光免疫定量分析仪;(b)日光灯下,阴性、阳性低值、阳性高值样本的检测条;(c)荧光灯下,阴性、阳性低值、阳性高值样本的检测条

    Figure  6.  (a) MAYA fluorescence immunoquantitative analyzer; (b) Test strips for negative, low positive and high positive samples under fluorescent lamps; (c) Test strips for negative, low positive and high positive samples under fluorescent lamps

    表  1  SAA质控品定标的数据

    Table  1.   SAA quality control product calibration data

    ρ(SAA) /(mg·L−1) Fluorescene/(a.u.)
    Data 1Data 2Data average
    A LineB LineA LineB LineA LineB Line
    0 1145 9 1150 9 1147.5 9
    6.75 1260 109 1262 109 1261 109
    13.5 1355 193 1350 187 1352.5 190
    27 1540 354 1536 345 1538 349.5
    54 1881 638 1899 662 1890 650
    108 2638 1298 2619 1275 2628.5 1286.5
    216 3627 2022 3665 2038 3646 2030
    下载: 导出CSV

    表  2  本文和龙华医院测试临床样本SAA测值比较

    Table  2.   Comparation of SAA value between this study and Longhua Hospital

    Sampleρ(SAA)/(mg·L−1
    This studyLonghua HospitalDeviation/%
    11.611.72−6.40
    22.302.137.98
    33.473.75−7.47
    43.733.98−6.28
    511.8810.3814.45
    622.5820.898.09
    723.7326.63−10.89
    841.4936.2514.46
    959.7253.7611.09
    10115.14100.5214.54
    下载: 导出CSV
  • [1] LUO J, XIE Z, LAM J W, et al. Aggregation-induced emission of 1-methyl-1, 2, 3, 4, 5-pentaphenylsilole[J]. Chemical Communications, 2001, 18: 1740-1741.
    [2] QIAN J, TANG B Z. AIE luminogens for bioimaging and theranostics: From organelles to animals[J]. Chemistry, 2017, 3(1): 56-91. doi: 10.1016/j.chempr.2017.05.010
    [3] GU B, YONG K T, LIU B. Strategies to overcome the limitations of AIEgens in biomedical applications[J]. Small Methods, 2018, 2(9): 1700392. doi: 10.1002/smtd.201700392
    [4] QI J, SUN C, ZEBIBULA A, et al. Real-time and high resolution bioimaging with bright aggregation-induced emission dots in short-wave infrared region[J]. Advanced Materials, 2018, 30(12): 1706856. doi: 10.1002/adma.201706856
    [5] ZHANG Q, Yu P, Fan Y, et al. Bright and stable NIR-II J-Aggregated AIE dibodipy-based fluorescent probe for dynamic in vivo bioimaging[J]. Angewandte Chemie International Edition, 2021, 133(8): 4013-4019.
    [6] CHEN Y, Zhang W, Zhao Z, et al. An easily accessible ionic aggregation-induced emission luminogen with hydrogen-bonding-switchable emission and wash-free imaging ability[J]. Angewandte Chemie International Edition, 2018, 57(18): 5011-5015. doi: 10.1002/anie.201800772
    [7] NIU G, ZHANG R, SHI X, et al. AIE luminogens as fluorescent bioprobes[J]. TrAC Trends in Analytical Chemistry, 2020, 123: 115796.
    [8] WANG H, CHEN X, QI Y, et al. Aggregation-induced emission (AIE)-guided dynamic assembly for disease imaging and therapy[J]. Advanced Drug Delivery Reviews, 2021, 179: 114028.
    [9] MEI J, HUANG Y, TIAN H. Progress and trends in AIE-based bioprobes: a brief overview[J]. ACS Appliaed Materials & Interfaces, 2018, 10(15): 12217-12261.
    [10] MAO L, LIU Y, YANG S, et al. Recent advances and progress of fluorescent bio-/chemosensors based on aggregation-induced emission molecules[J]. Dyes and Pigments, 2019, 162(3): 611-623.
    [11] LIU S, FENG G, TANG B Z, et al. Recent advances of AIE light-up probes for photodynamic therapy[J]. Chemical Science, 2021, 12: 6488-6506.
    [12] ALAM P, LEUNG N L C, ZHANG J, et al. AIE-based luminescence probes for metal ion detection[J]. Coordination Chemistry Reviews, 2021, 429: 213693.
    [13] GRONDMAN I, PIRVU A, RIZA A, et al. Biomarkers of inflammation and the etiology of sepsis[J]. Biochemical Society Transactions, 2020, 48(1): 1-14. doi: 10.1042/BST20190029
    [14] CHANNAPPANAVAR R, PERLMAN S. Evaluation of activation and inflammatory activity of myeloid cells during pathogenic human coronavirus infection[J]. MERS Coronavirus, 2020, 2099: 195-204.
    [15] KIM S W, CHO I H, LIM G S, et al. Biochemical-immunological hybrid biosensor based on two-dimensional chromatography for on-site sepsis diagnosis[J]. Biosensors & Bioelectronics, 2017, 98: 7-14.
    [16] SHEN H, WANG J, LIU H, et al. Rapid and selective detection of pathogenic bacteria in bloodstream infections with aptamer-based recognition[J]. ACS Appliaed Materials & Interfaces, 2016, 8(30): 19371-19378.
    [17] YANG K, LI H Z, ZHU X, et al. Rapid antibiotic susceptibility testing of pathogenic bacteria using heavy-water-labeled single-cell raman spectroscopy in clinical samples[J]. Analytical Chemistry, 2019, 91(9): 6296-6303. doi: 10.1021/acs.analchem.9b01064
    [18] KIM H, LEE J U, SONG S, et al. A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarker [J]. Biosensors & Bioelectronics, 2018, 101: 96-102.
    [19] GILLMORE J D, LOVAT L B, PERSEY M R, et al. Amyloid load and clinical outcome in AA amyloidosis in relation to circulating concentration of serum amyloid A protein[J]. Lancet, 2001, 358: 24-29.
    [20] TAKASE H, TANAKA M, MIYAGAWA S, et al. Effect of amino acid variations in the central region of human serum amyloid A on the amyloidogenic properties[J]. Biochemical and Biophysical Research Communications, 2014, 444(1): 92-97. doi: 10.1016/j.bbrc.2014.01.029
    [21] SACK G H. Serum amyloid A: A review [J]. Molecular Medicine, 2018, 24: 46.
    [22] BANG Y J, HU Z , LI Y, et al. Serum amyloid A delivers retinol to intestinal myeloid cells to promote adaptive immunity[J]. Science, 2021, 373: 6561.
    [23] PIERI M, CIOTTI M, NUCCETELLI M, et al. Serum amyloid A protein as a useful biomarker to predict COVID-19 patients severity and prognosis[J]. International Immunopharmacology, 2021, 95: 107512.
    [24] 中国妇幼保健协会临床诊断与实验医学分会. SAA 单独和与 CRP 联合检测在儿童感染性疾病中的应用专家共识[J]. 检验医学, 2021, 36(7): 1673-8640.
    [25] LV Y J, HU Q L, HUANG R, et al. The diagnostic and therapeutic value of the detection of serum amyloid A and C-reactive protein in infants with rotavirus diarrhea[J]. International Journal of General Medicine, 2021, 14: 3611-3617.
    [26] LIU Q, LI Y, YANG F, et al. Distribution of serum amyloid A and establishment of reference intervals in healthy adults[J]. Journal of Clinical Laboratoty Analysis, 2020, 34(4): e23120.
    [27] SUPIANTO M, LEE S H, JHUNG S H, et al. Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1[J]. Microchimica Acta, 2021, 188: 386.
    [28] SMOLE U, GOUR N, PHELAN J, et al. Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity[J]. Nature Immunology, 2020, 21: 756-765.
    [29] SONG C, CHENG K, GUO J, et al. Combined detection of C-reactive protein and serum smyloid A based on up-conversion luminescent system for internet of medical things application[J]. IEEE Transactions on Nanotechnology, 2021, 20: 708-714.
    [30] 黄存存, 晏琦帆. 基于萘酸酐的水溶性荧光探针: 合成以及在细胞成像中的应用[J]. 华东理工大学学报(自然科学版), 2021, 47(5): 569-576.
    [31] GUO Z, SHAO A, ZHU W H. Long wavelength AIEgen of quinoline-malononitrile[J]. Journal of Materials Chemistry C, 2016, 4(14): 2640-2646. doi: 10.1039/C5TC03369A
    [32] GUO Z, YAN C, ZHU W H. High-performance quinoline-malononitrile core as a building block for the diversity-oriented synthesis of AIEgens[J]. Angewandte Chemie International Edition, 2020, 59(25): 9812-9825. doi: 10.1002/anie.201913249
    [33] 张洪涛, 吕睿, 陈敏. 分散共聚合制备PSt-AA-EGDMA功能性单分散微米级交联微球的研究[J]. 高等学校化学学报, 2004, 25(2): 366-371.
  • 加载中
图(6) / 表(2)
计量
  • 文章访问数:  20
  • HTML全文浏览量:  5
  • PDF下载量:  4
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-04-14
  • 网络出版日期:  2022-05-09

目录

    /

    返回文章
    返回